Associate Scientist III

Associate Scientist III, wanted by Insitro, Inc. in South San Francisco, CA. Establishing and scaling up robust differentiation of iPSCs into mature cell types relevant to human disease, with a priority on microglia and astrocyte differentiation across multiple patient donor lines; Working with both transcription factor driven and directed differentiation techniques; Adapting existing protocols to scaled production, followed by development of standardized quality control assays and cryopreservation of progenitor banks; Incorporating and executing established protocols to develop co-culture and tri-culture in vitro models; Performing cell line engineering of iPSCs using various transduction and transfection techniques such as piggy-back transposon, viral transduction, and lipofectamine for random integration of transcription factor-expression cassettes; Characterizing the development of protocols for in-vitro cellular models using various techniques, including flow cytometry, immunocyto